A unique feature of the Biotron, which strengthens its collaboration capabilities, is the capacity to provide researchers with real-time, worldwide, experimental management. At the core of the Biotron's scientific functionality will be its secure, high-speed, web-accessible imaging database and data analysis system providing state-of-the-art capacity in:
  • imaging equipment manipulation and data acquisition;
  • analytical instrument and research chamber manipulation and data acquisition;
  • data storage, sorting, archiving, processing, and analysis.
The system will allow image acquisition and analytical systems data to be stored, annotated, retrieved, processed, and accessed on a central server from the initiating workstation, Biotron computer labs, or remotely through the internet. Additionally, Biotron computer labs will provide users with collaboration tools such as video and web conferencing, and access to high-end image analysis and processing software.

The central computer lab is housed within a conference room and is connected to the Imaging suite. The computing and imaging areas serve as a focal point for the integration and international scope of the Biotron. Imaging technologies available in this suite feature the latest in confocal microscopy, digital light and fluorescence microscopy, transmission electron microscopy, and scanning electron microscopy. Contiguous with the Imaging suite is a spacious central laboratory for sample preparation, sectioning, coating as well as a cell tissue culture laboratory.

MICROSCOPY SERVICES

Zeiss LSM 5 Duo: The LSM 5 Duo is a unique, multipurpose confocal workstation, one of only a dozen in the world. It delivers true 5D experimental capability (X,Y,Z,Time and λ) by combining 3 instruments into one:

The LSM 510 multichannel point scanning confocal microscope offers ten optically tunable laser excitation wavelengths and a wide variety of beamsplitters and filters, providing excitation and detection capabilities ranging all the way from UV to near IR. The software setup and piezo-electric motorized stage allows the capture of detailed Z-stack images for precise 3D rendering and time series acquisition.

The LSM 5 Live employs a beam shaper for rapid laser LINE scanning, resulting in ultra high speed capture of live cell processes without the phototoxicity associated with common point scanning LSMs. Tracking biological molecules and physiological processes through photobleaching, photoactivation, photoconversion, uncaging and FRET is simple using the flexible and intuitive Zen Software. At 1010 fps (512x512 pixels) and 95% detection efficiency, the high speed detector can track processes lasting only a few microseconds.

The LSM 5 Meta can separate and acquire 32 spectral channels in only 1.2 seconds. A built-in spectral database, automatic component extraction, and revolutionary linear unmixing technology will allow the precise separation of fluorochromes: even those with extremely overlapping emission spectra such as GFP and FITC. It can completely eliminate crosstalk and autofluorescence in difficult samples; perform emission fingerprinting; or characterize unknown fluorescence signals through spectral analysis-even in the most demanding of dynamic samples.

Zeiss Axio Imager Z1 Microscope

A cornerstone in Biotron’s Imaging Module is the Z1 Imager by Zeiss. This compound microscope offers our users an ideal imaging solution with superb optics. Ergometric design means user comfort over long stints and the Z1 provides 8 channels of fluorescence, bright field, dark field, phase and DIC imaging. This fully digital Z1 Imager allows users to design and implement complex imaging projects with repeatability and control.

Zeiss SteReo Lumar V12 Microscope

The Lumar V12 is the first and only stereo microscope with the imaging quality of a compound light microscope. Our Lumar can provide a true stereo image at up to 125x magnification with sub-micrometer resolution, and do it in three fluorescent channels and in both transmitted and reflected light. This microscope is perfect for developmental biology projects, especially those looking at fluorescent protein expression (e.g. GFP, RFP) in whole organisms or tissues.

Hitachi 3400-N Variable Pressure Scanning Electron Microscope (VP-SEM)

Our Hitachi 3400-N is equipped with a turbo-molecular pump for quick pump down times and reliable high vacuum performance. This well equipped system offers users enhanced performance and capabilities including:
High Vacuum mode with both backscattered and secondary electron detectors; X-ray analysis; Peltier cooled stage and high resolution digital images. Capable of up to 300,000x magnification with better than 5nm resolution at 30 kV this versatile SEM is extremely user friendly. The 3400-N can handle specimens up to 200mm in diameter and offers fast (90 second) chamber pump down times for quick specimen exchange.
Variable Pressure Mode (to 280 Pa) adds a special Environmental Secondary electron detector to the list. In Variable Pressure mode we can image ‘wet’ or ‘oily’ samples without standard SEM processing protocols such as critical point drying or metal coating. Both X-ray analysis and high resolution digital image capture are available in the VP mode.

Philips CM 10 Transmission Electron Microscope

The extremely capable CM 10 is a user friendly instrument. Ours has been upgraded with a digital imaging system; it sports a LaB6 filament for greater beam stability and brightness. With up to 100Kv accelerating voltage, the CM 10 is an excellent system for both biological and material imaging.

Philips 420 Transmission Electron Microscope

The Philips 420 is equipped with a LaB6 filament and has an accelerating voltages up to 120Kv. Images are captured on film and later digitized. The instrument is equipped with a EDAX Genesis X-ray microanalysis system for materials characterization. The instrument is ideal for both biological and materials research.

For Microscopy enquiries contact: Karen Nygard at 519-661-2111 ext. 88061 or knygard@uwo.ca
Modules: Imaging
Modules: Imaging
Modules: Imaging
Modules: Imaging
Module: Imaging
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